Inhibitory Effect of Tricalcium Phosphate Sintered at Different Temperatures on Human Breast Cancer Cell Line MCF-7

Authors

  • Ali Akbar Zare Zare Breast Cancer Genetics Department, , Breast Cancer Research Center, ACECR , Tehran, Iran
  • Amir Seyfoori Breast Cancer Research Center, ACECR , Tehran, Iran and School of Metallurgy and Materials engineering , College of Engineering , University of Tehran, Tehran, Iran
  • Hasan Sanati Breast Cancer Research Center, ACECR , Tehran, Iran
  • Keivan Majidzadeh-A Breast Cancer Genetics Department, , Breast Cancer Research Center, ACECR , Tehran, Iran
  • Mehdi Rahmanian Breast Cancer Research Center, ACECR , Tehran, Iran
  • Seyed Morteza Naghib Breast Cancer Research Center, ACECR , Tehran, Iran and Nanotechnology Department, School of New Technologies, Iran University of Science and Technology (IUST), Tehran, Iran
Abstract:

Abstract Introduction: Achievement of new drugs with minimal side effects is a important goal in the cancer treatment due to irreversible side effects of conventional drugs. Tricalcium phosphates as natural bone components have unique characteristics including excellent biocompatibility, high biosorption and superior bioactivity. This research aimed at investigating the inhibitory effect of tricalcium phosphate (TCP) sintered at different temperatures. Methods: TCP nanoparticles (nTCP) were sintered at three temperatures of 700°C, 900°C and 1000°C, and their structural characterization was examined. Heat treatment of TCP at 900°C was found optimal due to its morphological properties, such as particle size and its crystallinity. The inhibitory effect of optimized nTCP sintered at 900°C was surveyed through in vitro tests. Results: Cell culture assay studies exhibited that such effect depended on the concentration of nTCP. Moreover, the results depicted that the effect was 80%, which could be attributed to the 50 mg.L-1 dose of nTCP. Conclusions: In lower concentrations, higher inhibitory effect of nTCP was observed. In comparison with hydroxyapatite, at low concentrations, anti-cancer properties of TCP were far greater than other calcium phosphates.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Cytotoxicity Effect of Cladribine on the MCF-7 Human Breast Cancer Cell Line

Cladribine, an analogue of deoxyadenosine, is highly toxic for both non-dividing and proliferating cells and has shown activity in the treatment of several malignancies. Therefore, the aim of the present study is to investigate the cytotoxicity effect of cladribine (2-CdA) on the breast cancer cell line, MCF-7 (estrogen receptor positive, ER+). MTT assay, annexin V-Fluorescein/PI and Hoechst 33...

full text

Evaluation of anticancer effect of hydroalcoholic extract of Stachys setifera on MCF-7 human breast cancer cell line

Abstract: Background and Aim: Breast cancer is the most common cancer among women. Given that plant compounds have been used to treat cancer for centuries. Despite some reports of antitumor effects on some species of this genus, the anticancer activity of Stachys setifera has not yet been reported. The aim of this study was to investigate the cytotoxic effect of hydroalcoholic extract of Stachy...

full text

Effect of Silibinin on Maspin and ERα Gene Expression in MCF-7 Human Breast Cancer Cell Line

Background and objective: According to reports, a serine protease inhibitor (Maspin) suppresses metastasis, invasion and angiogenesis in breast and prostate cancers. Silibinin is a natural polyphenolic flavonoid with anti-cancer activity. We assessed the effects of silibinin on cell viability, maspin and ERα gene expression in MCF-7 cell line. <s...

full text

cytotoxicity effect of cladribine on the mcf-7 human breast cancer cell line

cladribine, an analogue of deoxyadenosine, is highly toxic for both non-dividing and proliferating cells and has shown activity in the treatment of several malignancies. therefore, the aim of the present study is to investigate the cytotoxicity effect of cladribine (2-cda) on the breast cancer cell line, mcf-7 (estrogen receptor positive, er+). mtt assay, annexin v-fluorescein/pi and hoechst 33...

full text

Growth Inhibition and Apoptosis Induction of Salvia chloroleuca on MCF-7 Breast Cancer Cell Line

Fragrant species of the genus Salvia have been attributed many medicinal properties, which include anticancer activity. In the present study, cytotoxic properties of total methanol extract of Salvia chloroleuca Rech. f. & Aellen and its fractions were investigated on MCF-7, a breast carcinoma cell line. Malignant and non-malignant cells were cultured in RPMI medium and incubated with different ...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 1  issue 1

pages  11- 14

publication date 2017-01

By following a journal you will be notified via email when a new issue of this journal is published.

Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023